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DsRed-Monomer is an ideal fusion tag because it is a true monomer and for this reason has been expressed as a fusion with a large panel of diverse proteins with diverse functions and subcellular locations. All the proteins localized properly, including actin and Golgi fusions, which have not performed well with tetrameric DsRed proteins.
The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins.
DsRed-Monomer is an ideal fusion tag because it is a true monomer and for this reason has been expressed as a fusion with a large panel of diverse proteins with diverse functions and subcellular locations. All the proteins localized properly, including actin and Golgi fusions, which have not performed well with tetrameric DsRed proteins.
The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins. The DsRed-Monomer fluorescent protein is extremely stable, allowing you to monitor fluorescence over extended periods of time. However, it is not as bright as mCherry or tdTomato.
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