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The CAR Receptor Booster renders cells that cannot usually be transduced with adenovirus susceptible to infection. It can also be used to increase adenoviral transduction efficiency in all cell types. The receptor-coated cell-derived nanovesicles combine with the plasma membrane of your target cells to temporarily elevate the density of the adenovirus receptor protein CAR on the cell surface.
The CAR Receptor Booster renders cells that cannot usually be transduced with adenovirus susceptible to infection. It can also be used to increase adenoviral transduction efficiency in all cell types. The receptor-coated cell-derived nanovesicles combine with the plasma membrane of your target cells to temporarily elevate the density of the adenovirus receptor protein CAR on the cell surface. For example, hematopoietic cells or CHO cells, which are usually resistant to adenoviral infection, can be transduced following a simple pretreatment with CAR Receptor Booster (see data images by expanding the Details arrow in the product table below).
How does it work?
The CAR Receptor Booster consists of a vial of concentrated cell-derived nanovesicles that are densely coated with the coxsackie virus and adenovirus receptor protein (CAR). When the booster vesicles are applied to your cells (10–20 µl per well of a 6-well plate for ~2 hr), they fuse with the plasma membrane, resulting in an increased level of the receptor on the cell surface. Your target cells can then be more efficiently transduced with recombinant adenovirus.
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