Thermo Fisher Scientific HPRT1 Polyclonal Antibody
HPRT1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며 항원 친화 크로마토그래피로 정제되었습니다. 4°C 단기, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 23–218 of HPRT (Uniprot ID: P00492) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.06 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153553 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, Molt-4, Raji, mouse liver
- Predicted reactivity: Rat (96%), Zebrafish (93%), Xenopus laevis (92%), Dog (96%), Pig (97%), Rabbit (98%), Chicken (94%), Chimpanzee (99%), Bovine (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The HPRT1 protein is a transferase that catalyzes the conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate.
This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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