Thermo Fisher Scientific PKC alpha/beta/gamma Polyclonal Antibody
PKC alpha/beta/gamma 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB, IHC, ICC, ELISA 등 다양한 실험에 사용 가능. Rabbit IgG 기반, Protein A로 정제된 액상 제품이며, 인간·마우스·랫 등 반응성 보유. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human PKC alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Protein Kinase C alpha (PKC alpha) is a 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases. These kinases are involved in a wide range of physiological processes including mitogenesis, cell survival, and transcriptional regulation.
PKC alpha is ubiquitously expressed and regulates various cellular functions such as proliferation, differentiation, development, migration, cell-cell adhesion, cell–extracellular matrix adhesion, and solute transport.
The activation loop threonine (Thr497 in PKC alpha) is phosphorylated by phosphoinositide-dependent kinase 1 (PDK1). This phosphorylation enables autophosphorylation of threonine 638 in the carboxy terminus, which is critical for regulating PKC alpha dephosphorylation and inactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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