Takara Quick and easy yeast transformation from liquid culture or culture plates

The Quick & Easy Yeast Transformation Mix allows you to perform non-library scale transformations in the budding yeast, S. cerevisiae, or the methylotrophic yeast, P. pastoris, with an easy-to-use and time-saving protocol.

The S. cerevisiae protocol can be completed in less than 1.5 hours. It allows for the transformation of yeast cells grown in liquid culture, or direct transformation of yeast colonies picked from culture plates.

The P. pastoris protocol can be completed in less than 30 minutes, starting from a saturated overnight culture.

Overview

• Simple, rapid protocols:
  • 1.5 hours for S. cerevisiae
  • 30 minutes for P. pastoris
• No need to prepare competent yeast cells
• Transform S. cerevisiae yeast cells from plates up to 1 month old

More Information

Applications

• Transformation of yeast cells grown in liquid culture
• Direct transformation of S. cerevisiae yeast colonies picked from culture plates

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.