Thermo Fisher Scientific DDX3 Polyclonal Antibody
DDX3 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합합니다. Human, Mouse, Rat에 반응하며 Protein G로 정제된 고순도 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1:200–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human ATP-dependent RNA helicase DDX3X protein (2–662 AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 3.565 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.03% Proclin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3093513 |
Target Information
The protein encoded by this gene belongs to the DEAD-box protein family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif and ATP-dependent RNA helicase activity. It exhibits high RNA-independent ATPase activity, and unlike most DEAD-box helicases, its ATPase activity is stimulated by both RNA and DNA. This protein functions in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export, while cytoplasmic functions involve translation, cellular signaling, and viral replication. Misregulation of this gene has been linked to tumorigenesis. A paralog exists on the Y chromosome, and pseudogenes are present on chromosome 4 and the X chromosome. Alternative splicing generates multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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