Thermo Fisher Scientific Phospho-Vimentin (Ser83) Polyclonal Antibody
인산화된 Vimentin(Ser83)을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 ELISA에 적합합니다. Human, Mouse, Rat에 반응하며, 세포질 내 단백질 검출에 활용됩니다. 친화 크로마토그래피로 정제되었으며, PBS/glycerol 완충액에 보관됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around Ser83 of human VIM (NP_0033712) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.35 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914914 |
Product Specific Information
- Positive test controls: HeLa, NIH/3T3, C6
- Subcellular location: Cytoplasm
- Immunogen sequence: QDSVD
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It plays a role in intracellular protein transport between the nucleus and plasma membrane.
During early cellular development, vimentin is co-expressed with desmin and later replaced by tissue-specific IFPs.
Vimentin is implicated in steroid synthesis regulation by serving as a storage network for cholesterol-containing lipid droplets.
Phosphorylation of vimentin leads to intermediate filament breakdown and cytoskeletal rearrangement, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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