Thermo Fisher Scientific HPV Type 16 E7 Recombinant Mouse Monoclonal Antibody (Nb4)
HPV Type 16 E7 단백질을 인식하는 Mouse Recombinant Monoclonal 항체. Western blot, ELISA, Neutralization 등 다양한 응용에 사용 가능. CHO 세포 발현, Protein A/G 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| ELISA (ELISA) | Assay-dependent | - |
| Neutralization (Neu) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Mouse / VHH-mFc |
| Expression System | CHO cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Nb4 |
| Immunogen | Recombinant HPV 16 E7/Protein E7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Product Specific Information
Compatible with Direct ELISA.
Target Information
The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15 kDa), zinc-binding phosphoproteins localized in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses.
The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A.
Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22–26) involved in binding to the tumour suppressor protein pRb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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