Thermo Fisher Scientific CTCF Recombinant Rabbit Monoclonal Antibody (BLR041F)
CTCF 단백질을 인식하는 재조합 토끼 단클론 항체로 Western blot, IHC, ICC, Flow cytometry, IP에 적합. 인간 및 생쥐 시료에 반응하며 HEK293 세포에서 발현됨. 고순도 정제 항체로 4°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 - 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:500 |
| Flow Cytometry (Flow) | 1 µL per 1 × 10⁶ cells |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR041F |
| Immunogen | Peptide representing a region between residues 650–700 of human CCCTC-binding factor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 7.8–8.6, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Target Information
Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain-containing, DNA-binding nuclear phosphoprotein involved in multiple aspects of gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation, and regulation of imprinted sites. CTCF binds the CCCTC motif on DNA using different combinations of zinc finger domains and interacts with HAT- and HDAC-containing complexes, influencing transcriptional activation or repression. It is involved in transactivation of the APPB gene, silencing of the c-myc gene, insulation of human beta-globin and DMI myotonic dystrophy loci, and imprinting control of IGF2 and H19 regions. Mutations in CTCF have been associated with invasive breast, prostate, and Wilms’ tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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