Thermo Fisher Scientific NFIX Polyclonal Antibody
NFIX 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 적합합니다. Human 시료 반응성이 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 장기 보관 시 -20°C에서 안정적입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human NFIX. Recombinant protein control fragment (Product #RP-104554) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2663319 |
Product Specific Information
Immunogen sequence:
SPSYYNINQVT LGRRSITSPP STSTTKRPKS IDDSEMESPV DDVFYPGTGR SPAAGSSQSS GWPNDVDAGS PRATASALHF PSTSIIQQSS PYFTHPTIRY HHHHGQDSLK EFVQFVCSDG SGQATGQHSQ RQAPPLPTGL SASD
Highest antigen sequence identity to the following orthologs:
- Mouse: 99%
- Rat: 78%
Target Information
NF-1 (also designated CTF) is a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Human NF-1 mRNA analysis reveals two forms arising from alternative splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain and activates transcription through a proline-rich C-terminal transactivation domain. It recognizes and binds the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element is found in the promoter regions of various eukaryotic genes, including Ha-Ras, alpha-globin, HSP70, GRP78, Histone H1, myelin basic protein, and Xenopus laevis vitellogenin gene promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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