Thermo Fisher Scientific Diphtheria Toxin Polyclonal Antibody

Applications

• ELISA (ELISA): Assay-dependent

Product Specifications

Product Specific Information

• PA1-7212 detects diphtheria toxin.
• PA1-7212 has been successfully used in ELISA procedures.
• The immunogen is purified diphtheria toxoid.
• For longer term storage, aliquot and freeze at -20°C.

Target Information

Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae.
The toxin causes diphtheria in humans by entering the cell cytoplasm and inhibiting protein synthesis through ADP-ribosylation of elongation factor-2 (EF-2), rendering it inactive.
The catalyzed reaction is as follows:

NAD⁺ + peptide diphthamide → nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide

The crystal structure of the diphtheria toxin homodimer has been determined to 2.5 Å resolution. It reveals a Y-shaped molecule composed of three domains:

1. Catalytic domain (Fragment A) – alpha + beta type fold
2. Transmembrane (TM) domain – nine alpha-helices, some involved in pH-triggered membrane insertion and translocation
3. Receptor-binding domain – flattened beta-barrel with jelly-roll topology

The TM and receptor-binding domains together constitute fragment B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.