Thermo Fisher Scientific Phospho-Vimentin (Ser56) Polyclonal Antibody
인산화된 Vimentin(Ser56)을 인식하는 Rabbit Polyclonal Antibody로, Western blot에 적합합니다. 인간 시료에 반응하며 항원 친화 크로마토그래피로 정제되었습니다. PBS 기반 완충액에 보존되며 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Phospho-Vimentin (Ser56) Polyclonal Antibody
Applications
- Western Blot (WB)
Tested Dilution: 1:500–1:3,000
Publications: - [References not provided]
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Vimentin (phospho Ser56). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736432 |
Product Specific Information
- Positive Control: 293T, HeLa, HeLa (100 ng/mL Nocodazole, 24 hr)
- Predicted Reactivity: Chimpanzee (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs.
Vimentin plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin induces breakdown of intermediate filaments and activates contractile events dependent on ATP and myosin light chain, leading to cytoskeletal changes that facilitate cholesterol transport to mitochondria and enhance steroid synthesis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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