Thermo Fisher Scientific Calretinin Polyclonal Antibody
Rabbit polyclonal antibody against calretinin for WB and IHC(P) applications. Recognizes recombinant full-length mouse calretinin. Suitable for mesothelioma as positive control. Supplied as liquid, unconjugated, stored at 4°C short term or -20°C long t...
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:25 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full-length mouse calretinin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2068364 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is mesothelioma.
Target Information
Calretinin is encoded by the CALB2 gene located on chromosome 16.
It belongs to the troponin C superfamily and contains six EF-hand structural motifs that enable calcium binding.
This protein is involved in calcium signaling, buffering, and modulation of neuronal excitability.
Calretinin is expressed in the mesothelium, mast cells, certain neural cells, hair follicles, and fat cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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