Thermo Fisher Scientific PREP Polyclonal Antibody
Human PREP 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보존됩니다. 연구용으로 사용되며 높은 종간 항원 동일성을 보입니다.
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Thermo Fisher Scientific PREP Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PREP. Recombinant protein control fragment (Product #RP-94645). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645933 |
Product Specific Information
Immunogen sequence:
LSIREGCDPV NRLWYCDLQQ ESSGIAGILK WVKLIDNFEG EYDYVTNEGT VFTFKTNRQS PNYRVINIDF RDPEESKWKV LVPEHEKD
Ortholog sequence identity:
- Mouse: 91%
- Rat: 86%
Target Information
p33cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important for progression from G1 to S-phase. Cyclin A-cdk2 levels are maximal at the G1/S transition, and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as a substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and localized to the nucleus during late G1-, S-, and G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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