Thermo Fisher Scientific ERCC1 Monoclonal Antibody (OTI1C10), TrueMAB
ERCC1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, Flow Cytometry에 적합. Human 및 Mouse 시료 반응. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. DNA 복구 관련 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C10 |
| Immunogen | Full length human recombinant protein of human ERCC1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5′-incision during DNA repair.
It acts with SLX4 in the first step of interstrand cross-link (ICL) repair and participates in processing anaphase bridge-generating DNA structures that can lead to cytokinesis failure.
ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks in conjunction with SLX4.
(Source: UniProt)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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