Thermo Fisher Scientific BCAT1 Monoclonal Antibody (OTI3F5), TrueMAB
BCAT1 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 IHC(P) 적용 가능. 사람, 개, 마우스 반응성. 동결건조 형태로 제공되며, PBS(8% 트레할로스) 버퍼에 보관. 연구용으로 세포 성장 관련 효소 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F5 |
| Immunogen | Full length human recombinant protein of human BCAT1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The BCAT1 gene encodes the cytosolic form of branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids, essential for cell growth.
Defects in branched-chain amino acid transamination are associated with hypervalinemia and hyperleucine-isoleucinemia.
A mitochondrial form of this enzyme is encoded by a separate gene, and mutations in either gene may contribute to these disorders.
Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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