Thermo Fisher Scientific Axl Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 33-449 of human AXL (NP_0687132) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AXL,uniProtId:P30530-1,ncbiNodeId:9606,antigenRange:33-449,antigenLength:894,antigenImageFileName:PA5-116782_AXL_P30530-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116782_AXL_P30530-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.89 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901413

Product Specific Information

Positive Samples: HeLa, SW620, HepG2

Immunogen sequence: EESPFVGNPG NITGARGLTG TLRCQLQVQG EPPEVHWLRD GQILELADST QTQVPLGEDE QDDWIVVSQL RITSLQLSDT GQYQCLVFLG HQTFVSQPGY VGLEGLPYFL EEPEDRTVAA NTPFNLSCQA QGPPEPVDLL WLQDAVPLAT APGHGPQRSL HVPGLNKTSS FSCEAHNAKG VTTSRTATIT VLPQQPRNLH LVSRQPTELE VAWTPGLSGI YPLTHCTLQA VLSDDGMGIQ AGEPDPPEEP LTSQASVPPH QLRLGSLHPH TPYHIRVACT SSQGPSSWTH WLPVETPEGV PLGPPENISA TRNGSQAFVH WQEPRAPLQG TLLGYRLAYQ GQDTPEVLMD IGLRQEVTLE LQGDGSVSNL TVCVAAYTAA GDGPWSLPVP LEAWRPGQAQ PVHQLVKEPS TPAFSWP

Target Information

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.