Thermo Fisher Scientific Alpha SNAP Polyclonal Antibody
Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능한 Rabbit Polyclonal Antibody. Human, Mouse, Rat 반응성. Protein A로 정제된 1 mg/mL 액상 항체로, -20°C에서 보관. Alpha SNAP 단백질 인식용 연구용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Alpha SNAP (amino acids 201–295) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion, and transcytotic vesicles-to-plasma membrane transport.
Vesicle-to-target membrane docking occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Alpha-SNAP (or beta-SNAP in brain) binds to this docking complex, mediating the binding of NSF and forming a 20S fusion particle. Once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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