Thermo Fisher Scientific EIF4E2 Monoclonal Antibody (OTI1F5), TrueMAB
EIF4E2 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot에 최적화되어 있습니다. 인간 유래 EIF4E2 전장 단백질을 면역원으로 사용하며, 동결건조 형태로 제공됩니다. 연구용으로 번역 개시 억제 관련 연구에 활용 가능합니다.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F5 |
| Immunogen | Full length human recombinant protein of human EIF4E2 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
EIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap during the early step of translation initiation.
Acts as a repressor of translation initiation and competes with EIF4E, preventing eIF4F complex assembly at the cap.
References: PubMed 9582349, 17368478, 25624349, 22751931.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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