Thermo Fisher Scientific Acetyl-Histone H3 (Lys4) Polyclonal Antibody
Histone H3의 Lys4 아세틸화 형태를 인식하는 폴리클로날 항체로, Western blot, IHC, Dot blot에 사용 가능. Rabbit IgG 기반, 항원 친화 크로마토그래피로 정제됨. PBS 버퍼에 안정화제 포함, 4°C 또는 -20°C 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Dot Blot (DB) | 1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide containing ART[Acetyl-K]QTAR in which acetylation site is located at lysine 4 of human histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: 3T3 Nuclear Extract
- Cellular location: Nucleus
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it binds DNA to form the nucleosome structure.
Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo various post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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