Thermo Fisher Scientific TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Monoclonal Antibody (TIMP2, 4477)
인간 TIMP2 단백질을 인식하는 마우스 단클론 항체로, IHC(P) 및 펩타이드 어레이에 적합합니다. 단백질 A/G로 정제된 액상 형태이며, 4°C에서 보관합니다. TIMP2는 MMP-2 및 MMP-9 억제에 관여하여 결합조직 유지 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TIMP2, 4477 |
| Immunogen | Recombinant full-length human TIMP2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
TIMP-1 and TIMP-2 are inhibitory enzymes of the matrix metalloproteinase (MMP) family, crucial for maintaining connective tissue integrity.
TIMP-2 binds strongly to both latent (pro) and active forms of 72 kDa Type IV collagenase (MMP-2/gelatinase A), and also interacts with the active form of 92 kDa Type IV collagenase (MMP-9/gelatinase B).
TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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