Thermo Fisher Scientific V5 Tag Monoclonal Antibody (E10/V4RR)
V5 태그가 부착된 융합 단백질을 인식하는 Mouse IgG1 단클론 항체. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 사용 가능. 단백질 A로 정제된 액상 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 권장. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 | View 42 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:2,000 | View 10 publications |
| Flow Cytometry (Flow) | – | View 2 publications |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | Assay-dependent | View 11 publications |
| Miscellaneous (PubMed) | – | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | E10/V4RR |
| Immunogen | GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10977225 |
Available Conjugate Formats
Product Specific Information
MA5-15253 recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins.
MA5-15253 may be stored at 4°C for short-term use. Add 0.05% sodium azide if desired. For long-term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or low-abundance proteins when specific antibodies are unavailable.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag typically includes all 14 amino acids (GKPIPNPLLGLDST), though shorter versions (IPNPLLGLD) have also been used.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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