Thermo Fisher Scientific V5 Tag Monoclonal Antibody (E10/V4RR)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

View 42 publications 42 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:2,000

View 10 publications 10 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

View 11 publications 11 publications

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

E10/V4RR

Immunogen

GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• DyLight 488
• DyLight 650
• DyLight 680
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10977225

Product Specific Information

MA5-15253 recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins.

MA5-15253 may be stored at 4° C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20° C, avoiding freeze/thaw cycles.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.