Thermo Fisher Scientific P2Y14 Monoclonal Antibody (5A3F1)
인간 P2Y14 단백질을 인식하는 마우스 단클론 항체로 다양한 면역실험(WB, IHC, ICC, Flow, ELISA)에 사용 가능. 고순도 Protein G 정제, 1 mg/mL 농도, PBS 및 sodium azide 포함. 연구용으로 GPCR 관련 신호전달 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A3F1 |
| Immunogen | Purified recombinant fragment of human P2RY14 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787442 |
Product Specific Information
Amino acid sequence of the immunogen is proprietary. This antibody has been tested in indirect ELISA.
Target Information
GPR105 (also known as P2Y14) has been reported to be expressed in brain. ESTs have been isolated from kidney, placenta, uterus, and heart/melanocyte/uterus libraries.
G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices and exist in both active and inactive forms.
An array of activating ligands participate in the conformation of GPCRs leading to signaling via G-proteins and downstream effectors. Studies have shown complex mechanisms of negative regulation of GPCRs, which have major implications for cellular physiology and pharmacological research.
Notable GPCR-targeted drugs include Zyprexa (Eli Lilly) and Claritin (Schering-Plough), both major products in mental health and allergy markets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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