Thermo Scientific™

Pierce™ Gaussia Luciferase Flash Assay Kit

The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of자세히 알아보기

The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

Features of the Gaussia Luciferase Flash Assay Kit:

• Sensitive—1000 times greater sensitivity allows utilization of smaller numbers of cells
• Cost effective—highest sensitivity assays result in decreased reagent consumption
• Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
• Time-saving—assays using secreted luciferases require minimal sample handling
• Automation-friendly—amenable to high throughput screens
• Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
• Safe—allows one to perform non-radioactive assays

This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing _cis_- regulatory elements and _trans_-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The signal produced by Gaussia luciferase shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions. The assay kit is optimized for use with our Gaussia Luc Vectors but is fully compatible with other Gaussia luciferases and derivatives that use coelenterazine as a substrate. (For glow assays, use our _Gaussia_-Dura Luc Vectors.)

More Product Data
• Highly sensitive multiplex luciferase reporter assays
• Luciferase assays in hard-to-transfect Jurkat cells
• Monitoring neuronal differentiation using multiplexed luciferase reporters
• Activation of the antioxidant response pathway by pesticide chemicals
• Versatile luciferases: microplate luminometers and flash luciferase assays

사양

유형Assay Kit

타겟Luciferase, Gaussia Luciferase

충분100 Luciferase Assays

수량100 Reaction Kit

어세이Reporter Enzyme, Luciferase Reporter Assay

호환 셀Mammalian Cells

검출 방법Bioluminescence

용도(장비)Luminometer (Microplate)

형식384-well plate, 96-well plate

라벨 유형Enzyme Labeled

기질 특성Chemical Substrate

기술Enhanced Chemiluminescence

기질 유형Luciferase Substrate

제품라인Pierce™

기질Coelenterazine

Unit SizeEach