Thermo Scientific™

pTK-Green Renilla Luc Vector for Luciferase Assays

The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under자세히 알아보기

The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pTK-Green Renilla Luc vector:

• Weak (TK) constitutive promoter for co-transfection and normalization
• Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

More Product Data
• Luciferase assays in hard-to-transfect Jurkat cells

Related Products
pMCS-Green Renilla Luc Vector for Luciferase Assays
pCMV-Green Renilla Luc Vector for Luciferase Assays

사양

설명pTK-Green Renilla Luc Vector

유형Luc Vector

클로닝 방법Restriction Enzyme/MCS

배달 유형Transfection

수량10 μg

리포터 유전자Green Renilla Luciferase

선택 제제(진핵)Puromycin

제품라인Pierce™

용도(애플리케이션)Reporter Assays

프로모터TK

Unit SizeEach