Thermo Fisher Scientific Fibronectin Monoclonal Antibody (16E5)
Fibronectin 단백질을 인식하는 Mouse monoclonal antibody (Clone 16E5). Western blot, ICC/IF, IP에 사용 가능. 인간 혈장에서 정제된 단백질로 면역화됨. 액상 형태, 1 mg/mL 농도, -20°C 보관. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Immunoprecipitation (IP) | 5 µg | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16E5 |
| Immunogen | Protein purified from Human plasma |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_1957659 |
Product Specific Information
A suggested positive control for this product is human plasma.
Target Information
Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230–250 kDa. In the extracellular matrix of connective tissues and vessels, fibronectin exists as an insoluble, cross-linked protein forming high molecular mass polymers. It is abundant during embryonic development and tissue remodeling and also found as a soluble plasma protein.
Fibronectin consists of repeating modules forming domains that interact with fibronectin itself, other matrix components such as collagen and heparin, and cell surface receptors like integrins. It plays key roles in cell adhesion, migration, embryogenesis, wound healing, blood coagulation, host defense, and metastasis.
Fibronectin expression is elevated in non-small cell lung carcinoma, promoting tumorigenicity and resistance to apoptosis. The fibronectin gene undergoes alternative splicing, potentially producing up to 20 transcript variants, though not all full-length variants have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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