Thermo Fisher Scientific beta Amyloid (1-40) Monoclonal Antibody (32A1)

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

A suggested positive control for this product is Bosc23 cells transfected with Amyloid beta 40.

Target Information

Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer’s disease. Abeta peptide is 40–43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process.

The first step involves cleavage of the extracellular, amino-terminal domain of beta APP by beta-secretase (BACE), an aspartyl protease synthesized as a propeptide and activated by furin. Beta APP cleavage by mature BACE results in secretion of a segment of beta APP and a membrane-bound remnant.

The remnant is processed by gamma-secretase, a multi-subunit complex containing presenilin-1 and -2 as central components. The transmembrane glycoprotein nicastrin associates with presenilins and binds to the carboxyl-terminus of beta APP, modulating amyloid beta peptide production.

Abeta is an extracellular filamentous protein component of amyloid cores and neuritic plaques, also found in neurofibrillary tangles. Alzheimer’s disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down’s syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy), and the Guam Parkinson-Dementia complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.