Thermo Fisher Scientific OCT4 Monoclonal Antibody (GT486)
OCT4 단클론 항체(GT486)는 인간 및 생쥐에 반응하는 연구용 항체로, 줄기세포 표지자 분석에 적합합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제된 고순도 제품입니다.
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Thermo Fisher Scientific OCT4 Monoclonal Antibody (GT486)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Flow Cytometry (Flow) | 1:50–1:200 | View 1 publication |
| Immunoprecipitation (IP) | 1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT486 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Oct4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787090 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Cat (89%), Pig (88%), Rabbit (85%), Rhesus Monkey (97%), Chimpanzee (100%), Bovine (85%).
- Positive Control: NT2D1, human ESC, mouse ESC, HeLa transfected recombinant OCT3/4 protein.
- Centrifuge briefly prior to opening the vial.
Target Information
POU5F1, also known as Oct-4, is a maternally expressed octamer-binding transcription factor crucial for early embryonic development. It plays a key role in maintaining pluripotency and is used in stem cell research and regenerative medicine. Expression of POU5F1 along with Sox2, Klf4, Lin28, and c-Myc enables the generation of induced pluripotent stem (iPS) cells without viral vectors, improving safety for potential therapeutic applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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