Thermo Fisher Scientific Human FGF-4 Recombinant Protein, PeproTech
인간 FGF-4 재조합 단백질로, 세포 성장 및 분화 연구에 사용됩니다. E. coli 발현 시스템에서 생산되며 ≥95% 순도를 보장합니다. 세포 증식 분석에서 높은 생물학적 활성을 나타냅니다. 줄기세포, 발생학, 암 연구에 적합합니다.
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Applications
Western Blot (WB)
- Assay-dependent
ELISA
- Assay-dependent
Functional Assay
- Assay-dependent
In vitro Assay
- Assay-dependent
- View 58 publications
Miscellaneous PubMed
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published species | Cat, Chicken, Human, Mouse, Pig, Rat |
| Expression System | E. coli |
| Amino Acid Sequence | GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL |
| Molecular Weight | 19.7 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥95% by SDS-PAGE gel and HPLC analyses |
| Endotoxin Concentration | <1 EU/µg |
| Activity | Determined by a cell proliferation assay using Balb/c 3T3 cells. Expected ED50 ≤10 ng/ml (≥1×10^5 units/mg) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
Product Specific Information
- Catalog No. 100-31-1MG is provided as 2 × 500 µg (100-31-500UG).
- Recombinant Human FGF-4 is a 19.7 kDa protein consisting of 182 amino acid residues.
- Shipped at ambient temperature.
- For storage, handling, and reconstitution details, refer to the lot-specific Certificate of Analysis.
Target Information
Fibroblast Growth Factor 4 (FGF4) is a member of the FGF family with broad mitogenic and cell survival activities. It plays essential roles in stem cell growth and survival during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 has strong oncogenic and angiogenic properties, being expressed in various vascularized tumors and adult mouse tissues such as testis, intestine, and brain.
Mouse studies indicate its involvement in bone morphogenesis and limb development via the Sonic Hedgehog (SHH) signaling pathway. FGF4 also regulates neural progenitor proliferation and neuronal differentiation. Recent findings highlight its role in promoting human embryonic stem cell growth and suggest a feedback inhibition mechanism by an FGF4 splice isoform that may facilitate differentiation in later developmental stages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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