Thermo Fisher Scientific E2F3 Polyclonal Antibody
E2F3 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot 및 IHC(Paraffin) 검증 완료. 인간 및 생쥐 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태. 세포 주기 조절 연구에 적합.
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Applications
- Western Blot (WB): 1–2 µg/mL
- Immunohistochemistry (Paraffin) (IHC (P)): 2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3. The immunogen is located within amino acids 150–200 of E2F3. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2890387 |
Product Specific Information
Positive controls that may be used include:
- Human Lymph node Tissue Lysate
- Human Lymphoid Tissue Slide
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins, and is also a target of the transforming proteins of small DNA tumor viruses.
E2F proteins contain several evolutionally conserved domains, including:
- DNA binding domain
- Dimerization domain (interaction with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain (embedded within transactivation domain)
E2F3, along with E2F1 and E2F2, has an additional cyclin binding domain and binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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