Thermo Fisher Scientific PAPOL A+B+G Polyclonal Antibody
Human, Mouse, Rat에 반응하는 Rabbit Polyclonal Antibody로 Western blot 및 IHC(Paraffin)에 적합. KLH 결합 합성 펩타이드로부터 유래된 면역원 사용. Protein A로 정제된 액상 항체로, 0.01M TBS 버퍼에 50% 글리세롤 및 1% BSA 포함. -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human PAPOLA/PAPOLB/PAPOLG |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Polyadenylation of the 3′ ends of eukaryotic mRNAs is a key event that takes place in the nucleus during mRNA maturation. This reaction includes endoribonucleolytic cleavage of the pre-RNA at the poly(A) site, leading to synthesis of the poly(A) tail at the 3′ end of the upstream cleavage product.
Poly(A) polymerase (PAP) activity is required for the adenosine addition reaction. The testis expresses PAP-beta (TPAP) in the cytoplasm of spermatogenic cells, and its adenosine addition function plays a critical role in male germ cell production. PAP-beta-deficient transgenic mice show impaired expression of haploid-specific genes necessary for spermatogenesis. The intronless gene encoding human PAP-beta maps to chromosome 7p22.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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