Thermo Fisher Scientific CD163 Monoclonal Antibody (ED2)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Paraffin sections require protein digestion pre-treatment using trypsin or pronase.
• Suggested positive control for IHC: liver.
• For FACS, use 10 µL of the working dilution to label 1×10⁶ cells in 100 µL.
• Low-endotoxin, preservative-free formulation (Endotoxin <0.01 EU/µg).
• Mouse anti-Rat CD163, clone ED2 recognizes rat ED2 cell surface glycoprotein (Dijkstra et al., 1985).

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, part of the scavenger receptor cysteine-rich superfamily, and acts as a receptor for the hemoglobin-haptoglobin complex. It protects tissues from hemoglobin-mediated oxidative damage and plays a role in iron recycling via endocytosis and heme breakdown.

CD163 is expressed on human monocytes and macrophages, particularly during late inflammation phases. It is found on all circulating monocytes and most tissue macrophages except those in mantle zones, germinal centers, interdigitating reticulum cells, and Langerhans cells. Expression is upregulated by IL-10, IL-6, and dexamethasone, while LPS and PMA induce shedding into plasma or supernatant.

CD163 binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner, showing higher affinity for multimeric haptoglobin (HP1F phenotype). It triggers intracellular signaling cascades involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.