Thermo Fisher Scientific TrxR2 Monoclonal Antibody (7B2)

Applications and Tested Dilution

Product Specifications

Product Specific Information

A suggested positive control for this product is HeLa cells.

Target Information

The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxidoreductases. All mammalian TrxRs are homologous to glutathione reductase in primary structure, including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-), but distinctively have a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence (-Gly-Cys-SeCys-Gly-).
TrxR is a homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site, and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to the C-terminal SeCys-containing redox center, which then reduces substrates such as thioredoxin.
Members of the TrxR family are 55–58 kDa in size and include three isoforms: cytosolic TrxR1, mitochondrial TrxR2, and TrxR3 (also known as Trx and GSSG reductase, TGR). TrxR plays a key role in protecting cells against oxidative stress and regulating redox-dependent mechanisms of transcription factors and various biological processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.