Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), NovaFluor Blue 510, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV, Violet, Yellow series
• eFluor 450, 506
• FITC, PE, PE-Cyanine5, PE-Cyanine7
• Super Bright series
• Functional Grade
• Request custom conjugation available

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with other CD3 subunits (gamma and delta), the epsilon chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking via immobilized UCHT1 initiates intracellular activation and proliferation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Use 5 µL (0.4 µg)/test for staining a cell sample in 100 µL final volume. Recommended cell number: 10^5–10^8 cells/test.

Compatibility Notice:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

Buffer Recommendation:
Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample to antibody cocktails/master mixes prior to combining with cells.

Technology Information:
NovaFluor conjugates utilize Phiton technology with engineered fluorescent signatures designed to minimize spillover and spread impacts.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

Target Information

The CD3 complex transduces antigen-recognition signals in T cells and regulates TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, encoded on chromosome 11. The CD3 antigen is found on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지