Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), NovaFluor Blue 555, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 4 µL (0.6 µg)/test

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Product Specifications

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Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV, Violet, Yellow series
• eFluor 450, 506
• FITC
• PE, PE-Cyanine5, PE-Cyanine7
• Super Bright series
• NovaFluor Blue, Red, Violet, Yellow variants
• Functional Grade
• Custom conjugation available

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Product Specific Information

Description:
The UCHT1 monoclonal antibody reacts with human CD3ε, a 20 kDa subunit of the TCR complex. Along with other CD3 subunits (γ, δ), the ε chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates intracellular signaling leading to activation and proliferation.

Applications Reported:
Flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use 4 µL (0.6 µg) per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• Each NovaFluor conjugate or kit ships with CellBlox™ Blocking Buffer.
  • Add 5 µL per sample when staining with NovaFluor conjugates.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Optical Properties:

• Excitation: 496 nm
• Emission: 555 nm
• Laser: 488 nm (Blue)

Technology:
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

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Target Information

The CD3 subunit complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes CD3γ, CD3δ, CD3ε, and CD3ζ subunits, encoded on chromosome 11. These subunits associate with intracellular signaling molecules via tyrosine-based motifs. CD3 is expressed on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells, but not on B or NK cells. Altered CD3 expression may be observed in autoimmune diseases. CD3 gene defects are associated with CD3 immunodeficiency.

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For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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