Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), NovaFluor Blue 555, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 4 µL (0.6 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV, Violet, Yellow series
• eFluor 450, 506
• FITC
• PE, PE-Cyanine5, PE-Cyanine7
• Super Bright series
• NovaFluor Blue, Red, Violet, Yellow variants
• Functional Grade
• Custom conjugation available

Product Specific Information

Description:
The UCHT1 monoclonal antibody reacts with human CD3ε, a 20 kDa subunit of the TCR complex. Along with other CD3 subunits (γ, δ), the ε chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates intracellular signaling leading to activation and proliferation.

Applications Reported:
Flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use 4 µL (0.6 µg) per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• Each NovaFluor conjugate or kit ships with CellBlox™ Blocking Buffer.
  • Add 5 µL per sample when staining with NovaFluor conjugates.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Optical Properties:

• Excitation: 496 nm
• Emission: 555 nm
• Laser: 488 nm (Blue)

Technology:
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

Target Information

The CD3 subunit complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes CD3γ, CD3δ, CD3ε, and CD3ζ subunits, encoded on chromosome 11. These subunits associate with intracellular signaling molecules via tyrosine-based motifs. CD3 is expressed on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells, but not on B or NK cells. Altered CD3 expression may be observed in autoimmune diseases. CD3 gene defects are associated with CD3 immunodeficiency.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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