Thermo Fisher Scientific Mouse IgG2a kappa Isotype Control (eBM2a), Brilliant Ultra Violet 496, eBioscience

Applications

• Flow Cytometry (Flow): Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

Description:
This monoclonal mouse IgG2a kappa antibody is used as an isotype control for mouse IgG2a antibodies.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood and mouse splenocytes. Use at the same concentration as the experimental antibody.

Fluorochrome Information:
Brilliant Ultra Violet™ 496 (BUV496) is a tandem dye that emits at 496 nm and is intended for use on cytometers equipped with a 355 nm ultraviolet laser. Ensure the instrument can detect this fluorochrome.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use one of the following buffers to minimize non-specific interactions:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vials and stained samples from light.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)
  for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.

Excitation: 350 nm
Emission: 496 nm
Laser: Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Mouse IgG2a is a subtype of immunoglobulin G produced by B cells in response to antigens. It plays a key role in adaptive immune responses, including:

• Opsonization
• Complement activation
• Antibody-dependent cell-mediated cytotoxicity (ADCC)
• Regulation of immune responses such as T-cell activation and B-cell inhibition

Widely used in:

• ELISA
• Western blotting
• Immunohistochemistry
• Immunotherapy and vaccine research

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available upon request

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