Thermo Fisher Scientific ASAP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ASAP1. Recombinant protein control fragment (Product #RP-104260). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ASAP1,uniProtId:Q9ULH1-1,ncbiNodeId:9606,antigenRange:957-1078,antigenLength:1129,antigenImageFileName:PA5-61770_ASAP1_Q9ULH1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-61770_ASAP1_Q9ULH1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2638266

Product Specific Information

Immunogen sequence: PKPGELPPKP QLGDLPPKPQ LSDLPPKPQM KDLPPKPQLG DLLAKSQTGD VSPKAQQPSE VTLKSHPLDL SPNVQSRDAI QKQASEDSND LTPTLPETPV PLPRKINTGK NKVRRVKTIY DC

Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 92%.

Target Information

ASAP1, also known as Development and differentiation enhancing factor 1, is a protein with two ANK repeats, an Arf-GAP domain, a PH domain and a SH3 domain. ASAP1 is a homodimer known to interact with SRC and CRK and its activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2). It possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. It may coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2 and may also function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. It is widely expressed in most of the tissues. ASAP1 is a protooncogene involved in invasive breast cancer and in other types of invasive and malignant tumors, such as uveal melanomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.