Thermo Fisher Scientific ID3 Monoclonal Antibody (OTI15F4), TrueMAB
ID3 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. HEK293T 세포에서 발현된 full-length human ID3 단백질을 면역원으로 사용. 고순도 Affinity chromatography 정제, -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:400 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI15F4 |
| Immunogen | Full length human recombinant protein of human ID3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.59 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3, and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA, and thus ID-type proteins negatively regulate DNA binding of bHLH proteins.
ID1 inhibits DNA binding of E12 and Myo D, functioning to inhibit muscle-specific gene expression. Under conditions favoring muscle cell differentiation, ID protein levels decrease, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, thereby activating myogenic differentiation. Expression of each ID protein is strongly dependent on growth factor activation, and reduction of ID mRNA levels by antisense oligonucleotides leads to delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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