Thermo Fisher Scientific Calpain 7 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2,500-1:5,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Calpain 7. Recombinant protein control fragment (Product #RP-88629). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calpain 7,uniProtId:Q9Y6W3-1,ncbiNodeId:9606,antigenRange:29-135,antigenLength:813,antigenImageFileName:PA5-61260_Calpain_7_Q9Y6W3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-61260_Calpain_7_Q9Y6W3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2639247

Product Specific Information

Immunogen sequence: AVFYYKEAAQ ALIYAEMAGS SLENIQEKIT EYLERVQALH SAVQSKSADP LKSKHQLDLE RAHFLVTQAF DEDEKENVED AIELYTEAVD LCLKTSYETA DKVLQNK

Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 95%.

Target Information

Calpain proteinases are defined currently as papain-like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain-4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date (calpain 5, 6, 8, 9, 10, 11, 12, 13, 14, 15) are not known to require a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain-T in calpains 5 & 6, domain-N for calpain-7). Calpain-7 domain-N does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. In place of the EF hand calcium-binding domain IV, calpain-7 has a sequence most closely resembling domain-III, with unknown function. Calpain-7 is widely distributed, found in most tissues analyzed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.