Thermo Fisher Scientific MBD1 Recombinant Rabbit Monoclonal Antibody (12H8L4)
MBD1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot과 Immunocytochemistry에 적합합니다. 인간에 반응하며 원숭이, 소에서도 예측 반응합니다. Protein A로 정제되었으며 PBS buffer에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 12H8L4 |
| Immunogen | Protein corresponding to human MBD1 [aa400–aa605] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809233 |
Product Specific Information
This antibody is predicted to react with Monkey and Bovine.
Target Information
Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA. It acts as a transcriptional repressor by binding CpG islands in promoters where DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG produced by DNA damage from methylmethanesulfonate (MMS). MBD1 plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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