Thermo Fisher Scientific E2F3 Polyclonal Antibody
E2F3 단백질을 표적으로 하는 Thermo Fisher Scientific의 rabbit polyclonal antibody. WB, IHC, ICC, ELISA 등 다양한 응용 가능. 고순도 Protein A 정제, 1 mg/mL 농도, 안정적 액상 형태로 연구용 적합.
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Thermo Fisher Scientific E2F3 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human E2F3 (amino acids 381–465) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins. It is also a target of transforming proteins of small DNA tumor viruses.
E2F proteins contain several evolutionarily conserved domains, including:
- DNA binding domain
- Dimerization domain (interacts with DP transcription factors)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain embedded within the transactivation domain
E2F3, along with E2F1 and E2F2, has an additional cyclin-binding domain and binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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