Thermo Fisher Scientific HSPBP1 Monoclonal Antibody (OTI1F3), TrueMAB
HSPBP1 단백질을 인식하는 Mouse IgG2b 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합. 인간 시료 반응성. 무보존제 동결건조 형태로 제공되며, 재구성 및 결합 실험에 용이.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F3 |
| Immunogen | Full length human recombinant protein of human HSPBP1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
HspBP1 is an Hsp70-interacting protein initially isolated from a human heart cDNA library via yeast two-hybrid system. It binds the ATPase domain of Hsp70, inhibiting its chaperone activity. HspBP1 also promotes nucleotide dissociation from Hsc70 and can alter the conformation of the Hsp70 ATPase domain, thereby regulating Hsp70 activity that requires ATPase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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