Thermo Fisher Scientific CD140a (PDGFRA) Monoclonal Antibody (APA5), eBioscience

Thermo Fisher Scientific CD140a (PDGFRA) Monoclonal Antibody (APA5), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• APC
• APC-eFluor 780
• Biotin
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation available

Product Specific Information

The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet-derived growth factor receptor (PDGF receptor). PDGFRα is a receptor tyrosine kinase that forms dimers on the cell surface upon ligand binding and phosphorylates substrates. Dimers may consist of alpha/alpha, beta/beta, or alpha/beta forms, serving as substrates for kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells in adult mouse tissues.

Applications Reported: APA5 has been used for flow cytometric analysis, immunoblotting (WB), immunohistochemical staining of frozen sections, and ligand blocking assays (use Functional Grade purified APA5 for functional assays).

Applications Tested: Tested by flow cytometric analysis of NIH/3T3 cells. Recommended ≤1 µg/test (100 µL final volume). Optimal antibody titration suggested.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

PDGFRA encodes a cell surface receptor tyrosine kinase binding platelet-derived growth factors, which act as mitogens for mesenchymal-origin cells. The receptor forms homodimers or heterodimers (α/β) depending on ligand identity. Knockout mouse studies show PDGFRA is vital for kidney development; heterozygous mice exhibit kidney defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.