Thermo Fisher Scientific CD140a (PDGFRA) Monoclonal Antibody (APA5), eBioscience
Mouse CD140a (PDGFRA) 단백질을 인식하는 Rat monoclonal antibody로, Flow Cytometry, Western Blot, Immunohistochemistry 등에 사용 가능. 비결합형 액상 포맷이며, 높은 순도(>90%)와 안정적 저장 조건(4°C)을 제공. 연구용 전용 제품.
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Thermo Fisher Scientific CD140a (PDGFRA) Monoclonal Antibody (APA5), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | 2 |
| Immunohistochemistry (IHC) | - | 42 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 3 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 4 |
| Immunocytochemistry (ICC/IF) | - | 15 |
| Flow Cytometry (Flow) | 1 µg/test | 67 |
| Immunoprecipitation (IP) | - | 1 |
| Functional Assay (Functional) | - | 1 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | APA5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467490 |
Available Conjugated Formats
- APC
- APC-eFluor 780
- Biotin
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation available
Product Specific Information
The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet-derived growth factor receptor (PDGF receptor). PDGFRα is a receptor tyrosine kinase that forms dimers on the cell surface upon ligand binding and phosphorylates substrates. Dimers may consist of alpha/alpha, beta/beta, or alpha/beta forms, serving as substrates for kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells in adult mouse tissues.
Applications Reported: APA5 has been used for flow cytometric analysis, immunoblotting (WB), immunohistochemical staining of frozen sections, and ligand blocking assays (use Functional Grade purified APA5 for functional assays).
Applications Tested: Tested by flow cytometric analysis of NIH/3T3 cells. Recommended ≤1 µg/test (100 µL final volume). Optimal antibody titration suggested.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
PDGFRA encodes a cell surface receptor tyrosine kinase binding platelet-derived growth factors, which act as mitogens for mesenchymal-origin cells. The receptor forms homodimers or heterodimers (α/β) depending on ligand identity. Knockout mouse studies show PDGFRA is vital for kidney development; heterozygous mice exhibit kidney defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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