Thermo Fisher Scientific ECD Polyclonal Antibody
ECD 단백질을 표적으로 하는 Rabbit Polyclonal Antibody로, WB 및 ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 연구용으로 p53 안정성 및 세포 주기 조절 연구에 적합.
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Thermo Fisher Scientific ECD Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ECD. Recombinant protein control fragment (Product #RP-89339). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.08 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2640870 |
Product Specific Information
Immunogen sequence:
DEDFECLDSD DDLDFETHEP GEEASLKGTL DNLKSYMAQM DQELAHTCIS KSFTTRNQVE PVSQTTDNNS DEEDSGTGES VMAPVDVDLN LVSNILESYS SQAGLAGPAS NLLQSMGVQL PDNTDHRPTS KP
Highest antigen sequence identity to the following orthologs:
- Mouse: 64%
- Rat: 69%
Target Information
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345).
May be involved in transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300.
May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932).
Involved in regulation of cell cycle progression and may disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839).
The cell cycle-regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270).
May play a role in regulation of pre-mRNA splicing (PubMed:24722212).
[UniProt]
Usage Notes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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