Thermo Fisher Scientific Phospho-PDHA1 (Ser293) Polyclonal Antibody
Phospho-PDHA1 (Ser293) 폴리클로날 항체로, 인간·생쥐·랫드 시료에 반응하며 Western blot과 ELISA에 적합합니다. Ser293 인산화 부위를 타깃으로 한 KLH 결합 합성 인산화 펩타이드로부터 제작되었습니다. 단백질 A로 정제된 액상 항체로, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO₂, linking glycolysis to the tricarboxylic acid (TCA) cycle. The PDH complex consists of multiple copies of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2), and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer composed of two alpha and two beta subunits. The gene encoding the E1 alpha 1 subunit contains the active site essential for PDH complex function. Mutations in this gene are linked to pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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