Thermo Fisher Scientific SF3A1 Monoclonal Antibody (OTI1F10), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The SF3A1 gene encodes subunit 1 of the splicing factor 3a protein complex. This complex, composed of subunits 1, 2, and 3, is essential for converting 15S U2 snRNP into the active 17S particle required for pre-mRNA splicing.
Subunit 1 belongs to the SURP protein family, containing SURP motifs involved in RNA binding. The N-terminal half includes tandem SURP motifs, while the C-terminal half contains a proline-rich region and a ubiquitin-like domain.
Binding studies indicate that the SURP motifs are necessary for interaction with subunit 3, while subunit 2 may interact through sequences C-terminal to the SURP motifs.
Alternative splicing produces multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.