Thermo Fisher Scientific Human IFN-gamma (ELISA Standard) Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human IFN-gamma, amino acids Gln24-Gln166 (Accession # NM_000619)

Molecular weight

20 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Description: Human IFN gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN gamma does not affect its biological activity.

Applications Reported: Recombinant Human IFN gamma ELISA Standard, Lyoph has been reported for use iin the Human IFN gamma ELISA Ready Set Go! (Product # 88-7316).

Applications Tested: The intended use of the Human IFN gamma RSG ELISA Lyophilized Standard is as a standard for Human IFN gamma ELISA Ready-SET-Go!® (Product # 88-7316-88). Typically the standard is run in a series of 7 doubling dilutions from the top standard. Please refer to the Certificate of Analysis included with each ELISA set for instructions on the proper reconstitution and dilution of the lyophilized standard.

Note: These are single-use vials. Use one time within 4 hours of reconstitution and discard. Reconstitute and dilute as suggested in the Certificate of Analysis or recovery may not be accurate.

Source: E. coli expressed amino acids Gln24-Gln166 of mature human IFN gamma accession # NM_000619.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.