Thermo Fisher Scientific Human IL-2 (ELISA Standard) Recombinant Protein, eBioscience

Applications

ELISA (ELISA)

• Assay-Dependent

Control (Ctrl)

• Assay-Dependent

Product Specifications

Product Specific Information

Human IL-2 (also known as TCGF) is a ~15 kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. It also acts on activated B cells, monocytes, NK, LAK cells, and oligodendroglial cells in vitro. In addition, IL-2 plays roles in hematopoiesis, tumor surveillance, and anti-inflammatory reactions, serving as a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.

Applications Reported:
Recombinant Human IL-2 ELISA Lyophilized Standard has been reported for use in the Human IL-2 Ready-Set-Go! ELISA kit (Product #88-7025).

Applications Tested:
Intended for use as a standard for the Human IL-2 Ready-Set-Go! ELISA kit (Product #88-7025-86). Typically, the standard is run in a series of 7 doubling dilutions from the top standard. Refer to the Certificate of Analysis included with each ELISA set for instructions on proper reconstitution and dilution of the lyophilized standard.

Note:
These are single-use vials. Use within 4 hours after reconstitution and discard. Reconstitute and dilute as suggested in the Certificate of Analysis to ensure accurate recovery.

Target Information

Interleukin 2 (IL-2) is an immunomodulatory cytokine important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes, and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by IL-4 and IL-7. Expression of the IL-2 gene in mature thymocytes is monoallelic, representing a unique regulatory mechanism for precise gene expression. Disruption of an IL-2-like gene in mice leads to an ulcerative colitis-like disease, suggesting its essential role in immune response to antigenic stimuli.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.