Thermo Fisher Scientific Mono-Methyl-Histone H3 (Lys9) Polyclonal Antibody
Mono-Methyl-Histone H3 (Lys9)에 특이적인 Rabbit Polyclonal Antibody로, Human, Mouse, Rat 시료에 반응합니다. Western blot, ICC/IF, IP 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 0.5 mg/mL 농도이며, 4°C 또는 -20°C에서 보관합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide containing AR[Me1-K]ST, where Me1-K corresponds to monomethyl lysine 9 of human histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: 3T3 Nuclear Extract
- Cellular location: Nucleus
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translational modifications of histones, such as methylation or acetylation of lysine and arginine residues and phosphorylation of serine or threonine, influence chromatin structure and gene expression. The N-terminal tail of histone H3 is subject to these modifications, impacting various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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