Thermo Fisher Scientific Goat anti-Mouse IgG3 Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
Alexa Fluor 488로 결합된 염소 유래 anti-Mouse IgG3 2차 항체로, WB, IHC, ICC, Flow Cytometry에 적합합니다. 높은 특이성과 낮은 교차 반응성을 가지며, 4°C에서 암소 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,500–1:5,000 |
| Immunohistochemistry (IHC) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
| Flow Cytometry (Flow) | 1–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse IgG3 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2535784 |
| Target | IgG3 |
| Cross Adsorption | Against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins, and mouse isotypes IgG1, IgG2a, IgG2b prior to conjugation |
| Antibody Form | Whole Antibody |
Additional Formats
- Alexa Fluor 594
- HRP
- Custom conjugation available upon request
Product Specific Information
- Product will be shipped at room temperature.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. These antibodies are useful for detection, sorting, or purification of their specific targets.
Secondary antibodies increase versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to enhance specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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